Afrotropical Research Articles

Species Diversity of the Leafhopper Tribe Paralimnini Distant (Hemiptera: Cicadellidae: Deltocephalinae) from Pakistan, with Description of Two New Genera

Abstract Paralimnini Distant, 1908, is a notable leafhopper tribe that exhibits a wide distribution across all biogeographical regions, namely the Palearctic, Nearctic, Afrotropical, Oriental, Neotropical, and Australian regions. Among these, the Oriental region demonstrates the highest diversity of Paralimnini, with a significant portion of this diversity remaining unexplored according to recent investigations. This study focuses on examining 10 species under seven genera of the Paralimnini leafhopper tribe found in Pakistan. A comprehensive morphological study is conducted, which includes the description of two new genera, Paralimnolus gen. nov. and Lalianlus gen. nov., with P. bicolor sp. nov. and L. bifurcates sp. nov. within the tribe. The study meticulously describes and provides illustrations of all the taxa based on careful observations of specimens obtained from Pakistan. Additionally, the study furnishes an updated checklist and a key to facilitate the identification of known and new Pakistani genera belonging to the tribe Paralimnini. The present findings shed light on the diversity and distribution patterns of the Paralimnini tribe in Pakistan, particularly emphasizing the junction of the Oriental and Palearctic regions in the northern parts, as these parts of the country serve as a significant reservoir of undocumented species. The thorough documentation and available identification resources provided in this study serve as valuable references for future investigations and contribute to our knowledge of the leafhopper fauna in Pakistan.

Low coverage of species constrains the use of DNA barcoding to assess mosquito biodiversity

Mosquitoes (Culicidae) represent the main vector insects globally, and they also inhabit many of the terrestrial and aquatic habitats of the world. DNA barcoding and metabarcoding are now widely used in both research and routine practices involving mosquitoes. However, these methodologies rely on information available in databases consisting of barcode sequences representing taxonomically identified voucher specimens. In this study, we assess the availability of public data for mosquitoes in the main online databases, focusing specifically on the two most widely used DNA barcoding markers in Culicidae: COI and ITS2. In addition, we test hypotheses on possible factors affecting species coverage (i.e., the percentage of species covered in the online databases) for COI in different countries and the occurrence of the DNA barcode gap for COI. Our findings showed differences in the data publicly available in the repositories, with a taxonomic or species coverage of 28.4–30.11% for COI in BOLD + GenBank, and 12.32% for ITS2 in GenBank. Afrotropical, Australian and Oriental biogeographic regions had the lowest coverages, while Nearctic, Palearctic and Oceanian had the highest. The Neotropical region had an intermediate coverage. In general, countries with a higher diversity of mosquitoes and higher numbers of medically important species had lower coverage. Moreover, countries with a higher number of endemic species tended to have a higher coverage. Although our DNA barcode gap analyses suggested that the species boundaries need to be revised in half of the mosquito species available in the databases, additional data must be gathered to confirm these results and to allow explaining the occurrence of the DNA barcode gap. We hope this study can help guide regional species inventories of mosquitoes and the completion of a publicly available reference library of DNA barcodes for all mosquito species.

HarvestNet: A Dataset for Detecting Smallholder Farming Activity Using Harvest Piles and Remote Sensing

Small farms contribute to a large share of the productive land in developing countries. In regions such as sub-Saharan Africa, where 80% of farms are small (under 2 ha in size), the task of mapping smallholder cropland is an important part of tracking sustainability measures such as crop productivity. However, the visually diverse and nuanced appearance of small farms has limited the effectiveness of traditional approaches to cropland mapping. Here we introduce a new approach based on the detection of harvest piles characteristic of many smallholder systems throughout the world. We present HarvestNet, a dataset for mapping the presence of farms in the Ethiopian regions of Tigray and Amhara during 2020-2023, collected using expert knowledge and satellite images, totalling 7k hand-labeled images and 2k ground-collected labels. We also benchmark a set of baselines, including SOTA models in remote sensing, with our best models having around 80% classification performance on hand labelled data and 90% and 98% accuracy on ground truth data for Tigray and Amhara, respectively. We also perform a visual comparison with a widely used pre-existing coverage map and show that our model detects an extra 56,621 hectares of cropland in Tigray. We conclude that remote sensing of harvest piles can contribute to more timely and accurate cropland assessments in food insecure regions. The dataset can be accessed through https://figshare.com/s/45a7b45556b90a9a11d2, while the code for the dataset and benchmarks is publicly available at https://github.com/jonxuxu/harvest-piles

New insights into the molecular phylogeny, biogeographical history, and diversification of Amblyomma ticks (Acari: Ixodidae) based on mitogenomes and nuclear sequences.

Amblyomma is the third most diversified genus of Ixodidae that is distributed across the Indomalayan, Afrotropical, Australasian (IAA), Nearctic and Neotropical biogeographic ecoregions, reaching in the Neotropic its highest diversity. There have been hints in previously published phylogenetic trees from mitochondrial genome, nuclear rRNA, from combinations of both and morphology that the Australasian Amblyomma or the Australasian Amblyomma plus the Amblyomma species from the southern cone of South America, might be sister-group to the Amblyomma of the rest of the world. However, a stable phylogenetic framework of Amblyomma for a better understanding of the biogeographic patterns underpinning its diversification is lacking. We used genomic techniques to sequence complete and nearly complete mitochondrial genomes -ca. 15 kbp- as well as the nuclear ribosomal cluster -ca. 8 kbp- for 17 Amblyomma ticks in order to study the phylogeny and biogeographic pattern of the genus Amblyomma, with particular emphasis on the Neotropical region. The new genomic information generated here together with genomic information available on 43 ticks (22 other Amblyomma species and 21 other hard ticks-as outgroup-) were used to perform probabilistic methods of phylogenetic and biogeographic inferences and time-tree estimation using biogeographic dates. In the present paper, we present the strongest evidence yet that Australasian Amblyomma may indeed be the sister-group to the Amblyomma of the rest of the world (species that occur mainly in the Neotropical and Afrotropical zoogeographic regions). Our results showed that all Amblyomma subgenera (Cernyomma, Anastosiella, Xiphiastor, Adenopleura, Aponomma and Dermiomma) are not monophyletic, except for Walkeriana and Amblyomma. Likewise, our best biogeographic scenario supports the origin of Amblyomma and its posterior diversification in the southern hemisphere at 47.8 and 36.8 Mya, respectively. This diversification could be associated with the end of the connection of Australasia and Neotropical ecoregions by the Antarctic land bridge. Also, the biogeographic analyses let us see the colonization patterns of some neotropical Amblyomma species to the Nearctic. We found strong evidence that the main theater of diversification of Amblyomma was the southern hemisphere, potentially driven by the Antarctic Bridge's intermittent connection in the late Eocene. In addition, the subgeneric classification of Amblyomma lacks evolutionary support. Future studies using denser taxonomic sampling may lead to new findings on the phylogenetic relationships and biogeographic history of Amblyomma genus.

Phylogeny and historical biogeography of the leafhopper subfamily Coelidiinae (Hemiptera: Cicadellidae) based on morphological and molecular data

AbstractThe phylogenetic relationships among major lineages of the globally distributed leafhopper subfamily Coelidiinae were reconstructed by analysis of 2903 nucleotide positions from two mitochondrial genes (COI and 16S), four nuclear genes (28S, H3, H2A and Wingless) and 102 discrete morphological characters, compiled for 86 species representing 52 genera within 9 coelidiine tribes broadly representative of the world fauna in addition to 12 outgroup taxa. Maximum likelihood and Bayesian analyses yielded well‐resolved phylogenetic estimates that were highly congruent with most branches receiving strong support. The results indicate Coelidiinae sensu stricto (=Coelidiinae sensu lato without Equeefini and Macroceratogoniini), Thagriini, Tharrini, Tinobregmini and Equeefini are monophyletic. However, the two largest tribes, Coelidiini and Teruliini, are paraphyletic, as well as Youngolidiini and most genera of Oriental Coelidiini. Fossil‐calibrated molecular divergence time analysis indicates that the Coelidiinae sensu stricto originated ca. 149 Ma, prior to the complete separation of the Gondwanan continents, and the tribe‐level lineages diverged between the Lower and Upper Cretaceous (92.77–138.03 Ma). The major lineages of Coelidiinae arose in the Oriental and Neotropical regions, and Oriental Coelidiini arose as a result of dispersal and colonisation from the Afrotropical region.

Synopsis of Neotropical Trogolaphysa Mills, 1938 (Collembola: Paronellidae) with Reduced Eye Number, and Description of Two New Troglobiontic Species from Belize

The genus Trogolaphysa comprises 81 described species distributed across the Neotropical and Afrotropical regions. In the Americas, just over half of the species found in subterranean habitats have a reduced eye number (0–5 eyes). Subterranean species are of interest as models to study the evolution of morphological adaptations. Many subterranean species of Trogolaphysa were described before the introduction of chaetotaxy as a diagnostic tool and thus remain incompletely described and diagnosed. To identify gaps in descriptions and facilitate the identification of newly collected forms, we provide standardized summary descriptions, species diagnoses, diagnostic tables, and a dichotomous key to the 33 Neotropical species of Trogolaphysa with reduced eye number. As a result of this synthesis, we describe two new troglobiontic species, Trogolaphysa reneaui n. sp. and Trogolaphysa welchi n. sp. from Belizean caves.

New insights into the systematics of the afrotropical Amblyomma marmoreum complex (Acari: Ixodidae) and the genome of a novel Rickettsia africae strain using morphological and metagenomic approaches

The Amblyomma marmoreum complex includes afrotropical species, such as Amblyomma sparsum, a three-host tick that parasitizes reptiles, birds, and mammals, and is a recognized vector of Ehrlichia ruminantium. However, the lack of morphological, genetic and ecological data on A. sparsum has caused considerable confusion in its identification. In this study, we used microscopy and metagenomic approaches to analyze A. sparsum ticks collected from a puff adder snake (Bitis arietans) in southwest Senegal (an endemic rickettsioses area) in order to supplement previous morphological descriptions, provide novel genomic data for the A. marmoreum complex, and describe the genome of a novel spotted fever group Rickettsia strain. Based on stereoscope and scanning electron microscopy (SEM) morphological evaluations, we provide high-quality images and new insights about punctation and enameling in the adult male of A. sparsum to facilitate identification for future studies. The metagenomic approach allowed us assembly the complete mitochondrial genome of A. sparsum, as well as the nearly entire chromosome and complete plasmid sequences of a novel Rickettsia africae strain. Phylogenomic analyses demonstrated a close relationship between A. sparsum and Amblyomma nuttalli for the first time and confirmed the position of A. sparsum within the A. marmoreum complex. Our results provide new insights into the systematics of A. sparsum and A. marmoreum complex, as well as the genetic diversity of R. africae in the Afrotropical region. Future studies should consider the possibility that A. sparsum may be a vector for R. africae.

Regional disparities in antenatal care utilization among pregnant women and its determinants in Ethiopia.

Antenatal care primarily focuses on health care checkups, the provision of advice on healthy behaviors, and the delivery of psychological, social, and emotional support for women with pregnancy. The national target set by the Ethiopian government is to achieve 95% of at least four ANC visits. Nevertheless, 43.11% of women had four or more checkups, according to the 2019 Ethiopian Demographic and Health Survey. Despite this achievement, antenatal care visits differ significantly between Ethiopian regions. Consequently, the purpose of this study was to assess regional disparities in pregnant women's utilization of antenatal care and its determinants in Ethiopia. We have used 2019 intermediate Ethiopian Demographic and Health Survey data for analysis. The analysis comprised a total of 3,917 weighted women age 15-49 who had a live birth in the 5 years preceding the survey. Poisson regression analysis was done using SAS software version 9.4. To show the strength and direction of the association, an incidence rate ratio with a 95% confidence interval was used. Variables with a p-value <0.05 were declared as significant factors associated with the number of ANC visits. In Ethiopia, the number of ANC visits differs between regions. With a mean of 4.74 (95% CI: 4.49, 4.99), Addis Ababa reported the highest percentage of ANC visits (82.7%), while the Somali Region reported the lowest percentage (11.3%) with a mean of 0.73 (95% CI: 0.57, 0.88). Maternal age, educational level, religion, household wealth index, place of delivery, and household size show significant associations with the number of antenatal care visits. In Ethiopia, there is the highest regional disparity in the number of ANC visits. The number of ANC visits was influenced by the mother's age, education, religion, household wealth index, place of delivery, and household size. Regarding the ANC visits, there should be initiatives that address the demands of pastoralist and agro-pastoralist communities to increase ANC utilization. As with many other health outcomes, education and low socio-economic status were associated with low ANC visit but these are tied to the overall social development of a country and are not immediately amenable to public health interventions.

Insularity and its impact on stingless bee honey properties: A case study in the Zanzibar Archipelago (Tanzania)

AbstractBackgroundIslands are known to host unique flowering plant assemblages visited by insular insects, including by social bees, a phenomenon thought to potentially impact the honey composition and associated medicinal applications. In this study, we characterized several physical–chemical parameters of honey produced by the stingless bee Meliponula (Axestotrigona) ferruginea with samples collected both from continental (Kenya) and insular (Tanzania) ecosystems.ResultsOur results show that stingless bees in islands produce a honey whose composition significantly differs from those produced in the continent, with a significantly higher concentration of bioactive phytochemicals.ConclusionOur results illustrate how insularity might drive different and unique compositional profiles in honeys and provide evidence that certain groups of compounds found in stingless bee honey from the Afrotropical region have potential therapeutic benefits with medicinal applications that largely remain to be explored.

New species and records of Afrotropical Campopleginae V. (Hymenoptera: Ichneumonidae)

Two new species of Afrotropical Campopleginae (Hymenoptera: Ichneumonidae) are described: Olesicampe africana sp. nov. from Guinea, and Sinophorus runei sp. nov. from South Africa, representing the first species of both genera described from the Afrotropical region, and, for the latter, the first species described from the Southern Hemisphere. Additionally, Dusona pauliani (Benoit, 1957), a species previously known from Réunion only, is reported for the first time from South Africa.

Phylogenomics of endemic Australian Ulopinae (Hemiptera: Cicadomorpha: Cicadellidae)

Ulopinae is a distinctive subfamily of leafhoppers that is widely distributed across the Afrotropical, Palearctic, Indomalayan and Australasian regions. The ulopine fauna of Australia is entirely endemic and includes two tribes of striking appearance, the Ulopini and Cephalelini. Knowledge of these groups is fragmentary and in many instances, no information is available beyond original descriptions. We assess the monophyly, phylogenetic placement and species-level diversity of the Ulopini genus Austrolopa. Phylogenetic analyses based on sequence data from target nuclear loci (18S, 28S, H2A and H3) and mitochondrial genomes (15 genes) for 23 membracoid taxa yielded congruent topologies. Our results provide strong evidence for the monophyly of Ulopinae and a clade consisting of Ulopini + Cephalelini. However, a non-monophyletic Cephalelini arises from within a polyphyletic Ulopini. Austrolopa was strongly recovered as monophyletic in all analyses, a result also supported by morphological features. The genus currently includes six species, three of which are described based on morphological and molecular data: Austrolopa botanica, sp. nov., Austrolopa rotunda, sp. nov. and Austrolopa sublima, sp. nov. A lectotype designation is provided for Austrolopa kingensis Evans, 1937, sp. reval. Our findings illustrate that the Australian Ulopinae is far more diverse than currently circumscribed and several species of Austrolopa are yet to be recognised. ZooBank: urn:lsid:zoobank.org:pub:1480285B-8F61-4659-A929-2B1EF3168868

Kola’s Kingdom: The Territory of Abasa (Western Somaliland) during the Medieval Period

AbstractDuring the thirteenth to sixteenth centuries, the territory of western Somaliland was integrated into a series of Muslim states which controlled large areas of the southeastern Horn of Africa. One of the ways this control manifested itself was in the emergence of a network of permanent settlements on the westernmost side of Somaliland and the neighboring Ethiopian region. Although 20 of these sites have been identified so far, our information about most of them is fragmentary at best. This paper presents a comprehensive study of the material, architectural and territorial context of three of these medieval sites: Abasa (Darbiyah Kola), Hasadinle, and Iskudarka. This paper analyzes the information they provide us for understanding some of the key themes of the history of the region, such as the strategies of state control, the process of Islamization, the relationships between nomads and urban dwellers, and the material expressions of hierarchization and social inequality.

Costs and resource needs for primary health care in Ethiopia: evidence to inform planning and budgeting for universal health coverage.

The Government of Ethiopia (GoE) has made significant progress in expanding access to primary health care (PHC) over the past 15 years. However, achieving national PHC targets for universal health coverage will require a significant increase in PHC financing. The purpose of this study was to generate cost evidence and provide recommendations to improve PHC efficiency. We used the open access Primary Health Care Costing, Analysis, and Planning (PHC-CAP) Tool to estimate actual and normative recurrent PHC costs in nine Ethiopian regions. The findings on actual costs were based on primary data collected in 2018/19 from a sample of 20 health posts, 25 health centers, and eight primary hospitals. Three different extrapolation methods were used to estimate actual costs in the nine sampled regions. Normative costs were calculated based on standard treatment protocols (STPs), the population in need of the PHC services included in the Essential Health Services Package (EHSP) as per the targets outlined in the Health Sector Transformation Plan II (HSTP II), and the associated costs. PHC resource gaps were estimated by comparing actual cost estimates to normative costs. On average, the total cost of PHC in the sampled facilities was US$ 11,532 (range: US$ 934-40,746) in health posts, US$ 254,340 (range: US$ 68,860-832,647) in health centers, and US$ 634,354 (range: US$ 505,208-970,720) in primary hospitals. The average actual PHC cost per capita in the nine sampled regions was US$ 4.7, US$ 15.0, or US$ 20.2 depending on the estimation method used. When compared to the normative cost of US$ 38.5 per capita, all these estimates of actual PHC expenditures were significantly lower, indicating a shortfall in the funding required to deliver an expanded package of high-quality services to a larger population in line with GoE targets. The study findings underscore the need for increased mobilization of PHC resources and identify opportunities to improve the efficiency of PHC services to meet the GoE's PHC targets. The data from this study can be a critical input for ongoing PHC financing reforms undertaken by the GoE including transitioning woreda-level planning from input-based to program-based budgeting, revising community-based health insurance (CBHI) packages, reviewing exempted services, and implementing strategic purchasing approaches such as capitation and performance-based financing.

New genera and species of Mantispoidea (Insecta, Neuroptera) from the mid-Cretaceous Kachin amber, Myanmar

Mantispoidea (Insecta, Neuroptera) are well-known for the hypermetamorphic larval development and for most adults having praying mantis-like raptorial forelegs. Currently, this superfamily comprises Berothidae (beaded lacewings) and three raptorial mantispoid families: Rhachiberothidae (thorny lacewings), Dipteromantispidae and Mantispidae (mantidflies or mantid lacewings). It includes about 530 extant species in 74 genera, and 143 extinct species in 97 genera from the Upper Triassic to the Miocene. Berothidae, well-known for some adults with scale-like setae on the female wings, currently includes 119 extant species in 26 genera, and 59 extinct species in 37 genera from the Upper Triassic to the Eocene. Rhachiberothidae represents the relictual group of Mantispoidea, comprising only 14 extant species across four genera, which are confined to the Afrotropical Region. However, the abundant fossil records (28 species and 21 genera), especially from the Cretaceous, highlight the past glory of this family. The Cretaceous dipteran-like family Dipteromantispidae, comprising ten species across nine genera, remains enigmatic in terms of its phylogenetic status. Mantispidae, the largest family within Mantispoidea, comprises approximately 395 extant species across 44 genera and 46 extinct species in 30 genera, ranging from the Lower Jurassic to the Miocene. This family is distinctive from other raptorial families for its highly mobile and variously elongated prothorax. Here, we describe several new genera and species from mid-Cretaceous Kachin amber, Myanmar: Three new species of Osmyloberotha Khramov, 2021 (O. dispersa sp. nov., O. chenzuyini Zhuo, Li &amp; Liu sp. nov. and O. magnimaculata sp. nov.) in Berothidae, with a detailed redescription of O. simpla Khramov, 2021; two new genera and three new species (Paraberothinae: Paraberothoides longispina Li, Zhang &amp; Liu gen. et sp. nov.; Subfamily unknown: Dicranoberotha zhangzhiqiae Zhuo, Li &amp; Liu gen. et sp. nov., Dicranoberotha liumohanae Zhuo, Li &amp; Liu gen. et sp. nov.) of Rhachiberothidae, three new species (Enigmadipteromantispa dilatata Li, Nakamine, Yamamoto &amp; Liu sp. nov., Kurtodipteromantispa relicta Li, Nakamine, Yamamoto &amp; Liu sp. nov. and Kurtodipteromantispa univenula sp. nov.) of Dipteromantispidae, as well as four new genera and five new species (Symphrasinae: Parvosymphrasites aploneura Li, Lin, Liu &amp; Wang gen. et sp. nov. and Proplega evelynae Zhuo, Li &amp; Liu gen. et sp. nov.; Subfamily unknown: Haplacantha robusta gen. et sp. nov., Haplacantha tenuis gen. et sp. nov. and Mesomantispoides felixoporcus gen. et sp. nov.) of Mantispidae. Sinuijumantispa So &amp; Won, 2022, previously classified within Drepanicinae, is now recognized as a stem group of Mantispidae, which is possibly related to Mesomantispoides gen. nov. or Mesomantispinae. Our new findings offer crucial insights into the taxonomy, morphological diversity and evolution of Mantispoidea.

Afrotropical Atrichops Verrall (Diptera, Athericidae) with description of a new species

Atrichops Verrall, 1909 of the Afrotropical Region are revised, and Atherix adamastor Stuckenberg, 1960, previously unplaced, is transferred to Atrichops based on morphological characters and feeding ecology. A new South African species, A. intermediussp. nov. is also described from Mpumalanga Province.

A new species of the planthopper genus Polychornum Gnezdilov, 2021 (Hemiptera: Caliscelidae: Ommatidiotinae) extends the distribution of the genus and tribe Augilini Baker to Africa

The caliscelid tribe Augilini Baker is recorded for the first time from African continent after Polychornum centroafricanum sp. n. described and illustrated from the Democratic Republic of Congo, collected in 1948. This is first representative of the genus Polychornum Gnezdilov, 2021 from the Afrotropical Region. Global distribution and host plants of the species of the tribe Augilini are discussed.

First discovery of the ant genus Eburopone Borowiec, 2016 (Hymenoptera, Formicidae, Dorylinae) in the Oriental realm, with description of a new species from Vietnam.

The doryline ant genus Eburopone Borowiec, 2016 currently contains only one valid species, E.wroughtoni (Forel, 1910) from southern Africa, with a considerable number of undescribed species awaiting formal description in the Afrotropical and Malagasy regions. In the present paper, Eburoponeeasoanasp. nov. is described based on workers and dealate queens from a colony series collected in an evergreen forest on the Dak Lak Plateau of Vietnam (Ea So Nature Reserve, Dak Lak Province). The worker of the new species is morphologically clearly distinguished from E.wroughtoni by the combination of following characteristics: i) frontal line distinct, extending a little beyond mid-length of cranium; ii) anterior (frontoclypeal) margins of torulo-posttorular complex not forming conspicuous lobes protruding over anterior clypeal margin in full-face view; iii) mandibles when closed in full-face view forming only a little space between anterior clypeal margin and mandibles; iv) promesonotal suture faint and inconspicuous; v) abdominal segment III in dorsal view distinctly wider than long, with lateral margins only feebly convex. This represents the first discovery of the genus Eburopone in the Oriental realm, revealing the disjunct distribution of the genus. A partial sequence of the mitochondrial COI gene (658 bp) is provided as a DNA barcode for the new species. A worker-based key to the doryline genera of the Oriental realm is also provided.

Immunohistochemistry-derived subtypes of breast cancer distribution in four regions of Ethiopia.

Different biological characteristics, therapeutic responses, and disease-specific outcomes are associated with different molecular subtypes of breast cancer (BC). Although there have been different studies on BC in the Ethiopian capital city of Addis Ababa, there have been few studies in other parts of the nation, and none have evaluated biological characteristics in other locations in the context of the extensive ethnic and genetic diversity found in Ethiopia. This study was carried out to evaluate the distribution of immunohistochemistry (IHC) subtypes of BCs throughout four Ethiopian regions. A total of 227 formalin-fixed paraffin-embedded (FFPE) tissue blocks were collected from tertiary hospitals in four Ethiopian regions between 2015 and 2021. The IHC staining was performed for subtyping, ER, PR, HER2, and Ki-67 proliferation markers. The mean age at diagnosis was 43.9 years. The percentage of ER and PR-negative tumors were 48.3% and 53.2%, respectively. The IHC subtypes showed the following distribution: 33.1% triple-negative breast cancer (TNBC), 27.6% luminal B, 25.2% luminal A, and 14.1% HER2 enriched. In multiple logistic regression analysis, grade III and HER2 positivity were associated with larger tumor size, and also originating from Jimma compared to Mekele. Patients with ER-negative, PR-negative, and TNBC were found in 48.3%, 53.2%, and 33.1% of cases, respectively, showing that half the patients could potentially benefit from endocrine treatment. A considerably high prevalence of TNBC was reported in our study, demanding additional research that includes genetic predisposition factors. Additionally, aggressive tumors were found in a high percentage of younger age groups, which must be considered when planning personalized treatment strategies.

An exceptional new weevil genus and species (Coleoptera: Curculionidae) from Tanzania

The new genus Borisius gen. nov. and the new species, Borisius korotyaevi sp. nov. are described and illustrated in the tribe Lixini from Tanzania in the Afrotropical region. The new genus can easily be recognized by the following combination of characters: antennal club greatly elongate and spindle-shaped, forehead with two prominent ridges triangularly diverging anteriad, pronotum constricted with two lateral projections, third elytral interstria strongly raised in basal fifth, humeri swollen, femora dentate.

Molecular-phylogenetic analyses of Ixodes species from South Africa suggest an African origin of bird-associated exophilic ticks (subgenus Trichotoixodes)

BackgroundAmong hard ticks (Acari: Ixodidae), the genus Ixodes comprises the highest number of species, which in turn are most numerous in the Afrotropical zoogeographic region. In South Africa extensive morphological studies have been performed on Ixodes species but only few reports included molecular analyses.MethodsIn this study, 58 Ixodes spp. ticks, collected from ten mammalian and eight avian host species in South Africa, were molecularly and phylogenetically analyzed. In addition, a newly collected sample of the Palearctic Ixodes trianguliceps was included in the analyses.ResultsAmong the ticks from South Africa, 11 species were identified morphologically. The majority of ticks from mammals represented the Ixodes pilosus group with two species (n = 20), followed by ticks resembling Ixodes rubicundus (n = 18) and Ixodes alluaudi (n = 3). In addition, single specimens of Ixodes rhabdomysae, Ixodes ugandanus, Ixodes nairobiensis and Ixodes simplex were also found. Considering bird-infesting ticks, Ixodes theilerae (n = 7), Ixodes uriae (n = 4) and ticks most similar to Ixodes daveyi (provisionally named I. cf. daveyi, n = 2) were identified. Molecular analyses confirmed two species in the I. pilosus group and a new species (I. cf. rubicundus) closely related to I. rubicundus sensu stricto. Phylogenetic trees based on concatenated mitochondrial or mitochondrial and nuclear gene sequences indicated that the subgenus Afrixodes forms a monophyletic clade with bird-associated exophilic ticks (subgenus Trichotoixodes). Ixodes trianguliceps clustered separately whereas I. alluaudi with their morphologically assigned subgenus, Exopalpiger.ConclusionsPhylogenetic analyses shed new lights on the relationships of Ixodes subgenera when including multiple sequences from subgenus Afrixodes and African as well as Palearctic species of subgenera Trichotoixodes and Exopalpiger. Subgenera Afrixodes and bird-associated Trichotoixodes share common ancestry, suggesting that the latter might have also originated in Africa. Regarding the subgenus Exopalpiger, I. alluaudi is properly assigned as it clusters among different Australian Ixodes, whereas I. trianguliceps should be excluded.Graphical